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{{/_source.additionalInfo}}Goat anti-Dog IgG (H&L), Unconjugated
(Cat#: AS16 3124)
Description
- Immunogen: purified dog normal IgG isolated from pooled dog serum
- Host: Goat
- Clonality: Polyclonal
- Purity: Protein G purified goat IgG in PBS pH 7.2.
- Format: Lyophilized
- Quantity: 1 ml
- Reconstitution: For reconstitution add 1 ml of sterile destilled water
- Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Reconstituted material can be stoored in 4°C up to 7 days.
- Tested applications: Immunoprecipitation (IP)
- Recommended dilutions: Immunoprecipitation, In immunoelectrophoresis use 2 ml or equivalent against 120 ml antiserum, In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 ml antiserum in a 3 mm diameter centre well and 2 ml serum samples (neat and diluted) in 2 mm diameter peripheral wells, Precipitating titre not less than 1:256 when tested against normal dog serum in agar block titration
- Confirmed reactivity: Dog IgG (heavy & light chains)
- Not reactive in: This antibody does not react with any non-Ig protein in dog serum, as tested by immunoelectrophoresis and double radial immunodiffusion
- Goat anti-Dog IgG (H&L), is a secondary antibody which binds to dog IgG heavy and light chain in immunological assays.
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