Anti-ACT 1,3,4,12 | Actin 1, 3, 4, 12 (clone mAB45a (5-15 H7C5))

(Cat#: AS16 3139)
AS16_3139
AS16_3139_1
Description
  • Immunogen: Full-length recombinant Arabidopsis thaliana Actin-1 (ACT1) expressed in E.coli, Uniprot: P0CJ46
  • Host: Mouse
  • Clonality: Monoclonal
  • Purity: IgG1 immunoglobulin purified on Protein G in 0.1 M Sodium Phosphate, pH 7.4, 0.15 M NaCl, 0.05% (w/v) sodium azide.
  • Format: Liquid
  • Quantity: 100 µg
  • Storage: Store at -20°C,Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
  • Tested applications: Western blot (WB)
  • Recommended dilutions: 1-2 µg/ml (WB)
  • Expected | apparent MW: 45 | 45 kDa
  • Confirmed reactivity: Arabidopsis thaliana!!AIR8!!Arabidopsis thaliana
  • Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Actin is a highly conserved protein and an essential component of cell cytoskeleton and plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth. Preferentially expressed in young and expanding tissues, floral organ primordia, developing seeds and emerging inflorescence.
  • Kandasamy, M.K. et al. (2012). Plant vegetative and animal cytoplasmic actins share functional competence for spatial development with protists. Plant Cell. 24, 2012 May;24(5):2041-57. doi: 10.1105/tpc.111.095281Kandasamy, M.K. et al. (2001). One plant actin isovariant, ACT7, is induced by auxin and required for normal callus formation. Plant Cell. Jul;13(7):1541-54Kandasamy, M.K. et al. (1999). The late pollen-specific actins in angiosperms. Plant Journal. Jun;18(6):681-91.
  • Exact working dilution needs to be determined by end user

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