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{{/_source.additionalInfo}}Anti-H3S10pT11p | Histone H3 (p Ser10, p Thr11)
(Cat#: AS16 3179)
Description
- Immunogen: KLH-conjugated synthetic peptide
- Host: Rabbit
- Clonality: Polyclonal
- Purity: Immunogen affinity purified serum.
- Format: Liquid
- Quantity: 50 µg
- Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
- Tested applications: Chromatin immunoprecipitation (ChIP), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)
- Recommended dilutions: 2-5 µg/million cells (ChIP), 1 : 200 (IF), 1 : 200 (IHC), 1 : 500 (WB)
- Expected | apparent MW: 15 kDa
- Confirmed reactivity: Caenorhabditis elegans, Human
- Not reactive in: No confirmed exceptions from predicted reactivity are currently known
- When confronted with DNA damage or the lack of certain kinases like Chk1 and Aurora1, histone H3 is phosphorylated at T11, causing a reduction in acetylation of the same histone at K9 and ultimately leading to a reduction of active transcription in affected cells. When the T11 phosphorylation is combined with a typical S10 phosphorylation that is critical in chromosome condensation in metaphase, the effect on transcriptional regulation is amplified further. Phosphorylation of H3S10 is decreased by T11 phosphorylation. Thus, detection of the combined modification is critical in determining transcriptional effects, especially when contrasted against single modifications of H3.
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