Anti-H3K27me3S28p | Histone H3 (trimethylated Lys27, p Ser28)

(Cat#: AS16 3184)
Description
  • Immunogen: KLH-conjugated synthetic peptide
  • Host: Rabbit
  • Clonality: Polyclonal
  • Purity: Immunogen affinity purified serum.
  • Format: Liquid
  • Quantity: 50 µg
  • Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
  • Tested applications: Chromatin immunoprecipitation (ChIP), Dot blot (Dot), Immunofluorescence (IF), Western blot (WB)
  • Recommended dilutions: 2-5 µg/million cells (ChIP), 1 : 500-1 : 1000 (Dot), 1 : 200 (IF), 1 : 50 (IHC), 1 : 500 (WB)
  • Expected | apparent MW: 15 kDa
  • Confirmed reactivity: Human
  • Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. Specifically, trimethylation of K27 is associated with gene silencing, whereas pS28 is associated with mitotis and immediate early genes.

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