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{{/_source.additionalInfo}}Anti-mCherry (rabbit antibody)
(Cat#: AS18 4179)
Description
- Immunogen: Recombinant full length mCherry expressed and purified from E. coli.
- Host: Rabbit
- Clonality: Polyclonal
- Purity: Immunogen affinity purified in PBS pH 7.4, with 3% trehalose.
- Format: Lyophilized
- Quantity: 100 µg
- Reconstitution: For reconstitution add 100 µl of sterile water
- Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
- Tested applications: Immunocytochemistry (ICC), Western blot (WB)
- Recommended dilutions: 1 : 250-1 : 500 (ICC), 1 : 500-1 : 1000 (WB)
- Confirmed reactivity: mCherry
- mCherry is derived from DsRed, a red fluorescent protein from so-called disc corals of the genus Discosoma. DsRed is a 223 amino acid ~28kDa protein similar in size and properties to GFP, hoever it produces a red rather than a green fluorochrome. mCherry in its monomeric form is useful for applications such as Förster Resonance Energy Transfer (FRET, also known as Fluorescence Resonance Energy Transfer). The protein is an engineered derivative of one of a family of proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals).
- Song et al. (2024). Substrate-induced condensation activates plant TIR domain proteins.Nature. 2024 Mar 13.doi: 10.1038/s41586-024-07183-9.
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