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{{/_source.additionalInfo}}Anti-FITC | fluorescein isothiocyanate, HRP-conjugated (monoclonal, Clone NI 239)
(Cat#: AS18 4215)
Description
- Immunogen: Highly purified fluorescein isothiocyanate isomer 1
- Host: Mouse
- Clonality: Monoclonal
- Purity: Purified IgG.1 in PBS pH 7.2, HRP conjugated.
- Format: Lyophilized
- Quantity: 200 µg
- Reconstitution: For reconstitution add 0.5 ml of sterile water
- Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Stort time storage at +4°C.
- Tested applications: ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (IHC) paraffin, Dot blot (Dot), Western blot (WB)
- Recommended dilutions: The optimum working dilution should be determined by titration by end user. 1:500 (ELISA), 1:50 to 1:200 (ICC, IHC), 1: 1000 (WB).
- Confirmed reactivity: FITC molecule
- FITC is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. FITC antibody is used to enhance the specific signal from a monoclonal antibody or a secondary antibody conjugated to FITC. The sensitivity of the FITC hapten-anti-hapten system makes it a useful alternative to other systems.
- This product is intended FOR RESEARCH USE ONLY.Peroxidase/IgG protein molar ratio (E/P) approximately 1.7.
Boca Scientific is your premiere source for high-quality, innovative solutions for Cell Biology, Molecular Biology, Immunology, genetics and other lab products and reagents. We bring leading-edge products from our own-line and around the world to laboratories in the US and Canada. Our goal is to offer excellent solutions to drive research and discoveries backed by superior customer support.
