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{{/_source.additionalInfo}}Anti-GFP | Green fluorescent protein (VENUS)
(Cat#: AS18 4227)
Description
- Immunogen: Full length, recombinat VENUS protein, expressed in E.coli, UniProt: P42212
- Host: Rabbit
- Clonality: Polyclonal
- Purity: Immunogen affinity purified serum in PBS pH 7.4.
- Format: Lyophilized
- Quantity: 50 µg
- Reconstitution: For reconstitution add 50 µl, of sterile water
- Storage: Lyophilized antibody can be stored at -20°C for up to 3 years. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
- Tested applications: Western blot (WB)
- Recommended dilutions: 1 : 1000 (WB)
- Expected | apparent MW: 26 kDa
- Confirmed reactivity: cell lysate overexoressing Venus protein fusion
- Not reactive in: No confirmed exceptions from predicted reactivity are currently known
- Green fluorescent protein (Venus) from Aequorea victoria (Jellyfish), can be mutated to emit at different wavelengths such as blue for BFP (when Tyr-66 is replaced by His), cyan for CFP (when Tyr-66 is replaced by Trp), and yellow for YFP (when THR-203 is replaced by Tyr). It is an energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer.
- Zhang et al. (2024). An epigenetically mediated double negative cascade from EFD to HB21 regulates anther development. Nat Commun. 2024 Sep 6;15(1):7796. doi: 10.1038/s41467-024-52114-x. Baiden et al. (2022). Heterologous expression of antimicrobial peptides S-thanatin and bovine lactoferricin in the marine diatom Phaeodactylum tricornutum enhances native antimicrobial activity against Gram-negative bacteria, Algal Research, Volume 69, 2023, 102927, ISSN 2211-9264, https://doi.org/10.1016/j.algal.2022.102927.
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