Anti-GFP | Green fluorescent protein (VENUS) - DyLight® 650 conjugated (40 µg)

(Cat#: AS18 4227-DL650)
AS18_4227-DL650
AS18_4227-DL650
Description
  • Immunogen: Full length, recombinant VENUS protein, expressed in E.coli, UniProt: P42212
  • Host: Rabbit
  • Clonality: Polyclonal
  • Purity: Immunogen affinity purified serum, in PBS pH 7.4, conjugated to DyLight® 650.
  • Format: Liquid in PBS pH 7,4.
  • Quantity: 40 µg
  • Storage: Store at 4°C for 12-18 months, A preservative may be added for long time storage up to 2 years. Spin briefly the tube before use.
  • Tested applications: Immunofluorescence (IF), Western blot (WB)
  • Recommended dilutions: To be determined by end user.
  • Expected | apparent MW: Depends upon fusion partner
  • Confirmed reactivity: VENUS
  • Not reactive in: No confirmed exceptions from predicted reactivity are currently known.
  • Green fluorescent protein (Venus) from Aequorea victoria (Jellyfish), can be mutated to emit at different wavelengths such as blue for BFP (when Tyr-66 is replaced by His), cyan for CFP (when Tyr-66 is replaced by Trp), and yellow for YFP (when THR-203 is replaced by Tyr), It is an energy-transfer acceptor, Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer.
  • To be added when available. Antibody released in May 2023.
  • DyLight® 650 has Amax = 652 nm, Emax = 672 nm. DyLight® is a registered trademark of Thermofisher Inc., and its subsidiaries.

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