Anti-Beta-Actin, (animal), mouse monoclonal

(Cat#: AS20 4381)
AS20_4381
AS20_4381
Description
  • Immunogen: Synthetic peptide derived from the N-terminal of the human beta-actin
  • Host: Mouse
  • Clonality: Monoclonal
  • Purity: IgG2b purified on Protein A n 10mM PBS, pH 7.2, with 1% BSA and 0.05% sodium azide.
  • Format: Lyophilized
  • Quantity: 100 µg
  • Storage: Store at -20°C for 3 years or more, Reconstitute with distilled water to desired concentration before use, Aliquot to avoid repeated freeze-thaw cycles, Store aliquots at 4°C for several days to weeks
  • Tested applications: Dot blot (Dot), ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
  • Recommended dilutions: 1: 1000 - 1: 5000 (WB), for other techniques end user needs to determine most optimal diltion
  • Expected | apparent MW: 42 kDa
  • Confirmed reactivity: Chicken, human, mouse, rabbit, rat
  • Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Beta-Actin is a conserved protein involved in cell motility, structure and integrity, tissue development and the development of organism. Actin is expressed at high levels in almost all tissues and cell lines. This makes it ideal to use as a loading control in Western blot.
  • 10mM PBS, pH 7,2, with 1% BSA and 0,05% sodium azide

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