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{{/_source.additionalInfo}}Anti-TGG1 | Myrosinase 1 ( BGL38)
(Cat#: AS20 4416)
Description
- Immunogen: BSA-conjugated peptide, derived from N-terminus of Arabidopsis thaliana TGG1, UniProt: P37702, TAIR: At5g26000
- Host: Rabbit
- Clonality: Polyclonal
- Purity: Total IgG. Protein A purified in PBS, 50% glycerol. Filter sterilized.
- Format: Liquid at 2 mg/ml.
- Quantity: 200 µg
- Storage: Store at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
- Tested applications: ELISA (ELISA), Immunogold (IG), Immunohistochemistry (IHC), Western blot (WB)
- Recommended dilutions: assay dependent (ELISA), 1: 1000 - 1: 2500 (IG), 1: 500 - 1: 1000 (IHC), 1: 1000-1: 3000 (WB)
- Expected | apparent MW: 61 | 77 kDa
- Confirmed reactivity: Arabidopsis thaliana
- Not reactive in: No confirmed exceptions from predicted reactivity are currently known
- In Brassicaceae, the enzyme myrosinase (beta-thioglucoside glucohydrolase, TGG) degrades glucosinolates to produce toxins like thiocyanates, isothiocyanates, nitriles, epithionitriles or oxazolidine- 2-thiones that deter herbivory. There are two TGG enzymes, TGG1 and TGG2, which have a redundant function. Cellular localization: vacuole.
- Farid et al. (2011). Arabidopsis thaliana alpha1,2-glucosyltransferase (ALG10) is required for efficient N-glycosylation and leaf growth. Plant J. 2011 Oct;68(2):314-25.doi: 10.1111/j.1365-313X.2011.04688.x. (Western blot)Shirakawa et al. (2010). Arabidopsis Qa-SNARE SYP2 proteins localized to different subcellular regions function redundantly in vacuolar protein sorting and plant development. Plant J. 2010 Dec;64(6):924-35.doi: 10.1111/j.1365-313X.2010.04394.x. (Western blot)Ueda et al. (2006). AtVAM3 is required for normal specification of idioblasts, myrosin cells. Plant Cell Physiol. 2006 Jan;47(1):164-75. doi: 10.1093/pcp/pci232. (Immunlocalization, Western blot).
- 19 amino acids of transit peptide are not present in the mature protein
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