Anti-NAI2 | TSA1-like protein (ER lumen marker)

(Cat#: AS20 4421)
AS20_4421
AS20_4421_1
Description
  • Immunogen: Purified recombinant NAI2 of Arabidopsis thaliana, residues 272-502 with a His tag, UniProt: Q9LSB4, TAIR: At3g15950
  • Host: Rabbit
  • Clonality: Polyclonal
  • Purity: Total IgG. Protein A purified in PBS, 50% glycerol. Filter sterilized.
  • Format: Liquid at 2 mg/ml.
  • Quantity: 200 µg
  • Storage: Store at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
  • Tested applications: Immunofluorescence (IF), Western blot (WB)
  • Recommended dilutions: 1: 1000 - 1: 3000 (IF), 1: 2000 - 1: 4000 (WB)
  • Expected | apparent MW: 85 | 120 kDa
  • Confirmed reactivity: Arabidopsis thaliana
  • Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • NAI2 TSA1-like protein is responsible for the ER body formation. Regulates the number and shape of the ER bodies and the accumulation of PYK10 in ER bodies, but is not involved in the expression of PYK10. Interacts directly or indirectly with MEB1 and MEB2. Expressed in roots. Detected in shoot apex. Induced by NAI1. The protein is localising to ER lumen.
  • Ueda et al. (2018).Endoplasmic Reticulum (ER) Membrane Proteins (LUNAPARKs) are Required for Proper Configuration of the Cortical ER Network in Plant Cells. Plant Cell Physiol. 2018 Oct 1;59(10):1931-1941. doi: 10.1093/pcp/pcy137. Yamada et al. (2013). Identification of two novel endoplasmic reticulum body-specific integral membrane proteins. Plant Physiol. 2013 Jan;161(1):108-20. doi: 10.1104/pp.112.207654. Yamada et al. (2008). NAI2 is an endoplasmic reticulum body component that enables ER body formation in Arabidopsis thaliana. Plant Cell . 2008 Sep;20(9):2529-40. doi: 10.1105/tpc.108.059345.
  • Signal peptide of 24 amino acids is removed from the mature protein

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