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{{/_source.additionalInfo}}Anti-SiR | Sulfite reductase [ferredoxin], chloroplastic
(Cat#: AS20 4424)
Description
- Immunogen: Purified full length, tag cleaved, recombinant Zea mays SiR, UniProt: O23813
- Host: Rabbit
- Clonality: Polyclonal
- Purity: Total IgG. Protein A purified in PBS, 50% glycerol. Filter sterilized.
- Format: Liquid at 4 mg/ml.
- Quantity: 200 µg
- Storage: Store at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
- Tested applications: ELISA (ELISA), Western blot (WB)
- Recommended dilutions: assay dependent (ELISA), 1: 1000 - 1: 5000 (WB)
- Expected | apparent MW: 70 kDa (Zea mays), 72 kDa (Arabidopsis thaliana)
- Confirmed reactivity: Arabidopsis thaliana, Pisum sativum, Zea mays
- Not reactive in: No confirmed exceptions from predicted reactivity are currently known
- Sulfite reductase (SiR) is an essential protein with sulfite reductase activity required in assimilatory sulfate reduction pathway during both primary and secondary metabolism and thus involved in development and growth. It is known as a DNA-binding protein that binds to both double-stranded and single-stranded DNA without significant sequence specificity to reversibly repress the transcriptional activity of chloroplast nucleoids by promoting DNA compaction and possibly regulate DNA replication. The sequence identity between maize and Arabidopsis SiR is 77%.
- Sato et al. (2001). The 70-kDa major DNA-compacting protein of the chloroplast nucleoid is sulfite reductase. FEBS Lett. 2001 Jan 5;487(3):347-50.doi: 10.1016/s0014-5793(00)02342-5. (Western blot, pea)Sakibara et al. (2000). Analysis of reductant supply systems for ferredoxin-dependent sulfite reductase in photosynthetic and nonphotosynthetic organs of maize. Plant Physiol. 2000 Mar;122(3):887-94.doi: 10.1104/pp.122.3.887. (Western blot, maize)
- This antibody is also recognizing recombinant SiR protein from Zea mays.
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