Anti-Glutamine synthetase (leaf,root)

(Cat#: AS20 4426)
AS20_4426
AS20_4426_1
Description
  • Immunogen: Purified full length, tag cleaved, recombinant Zea mays GS-1 (Glutamine synthetase, root isozyme 1) UniProt: P38559
  • Host: Rabbit
  • Clonality: Polyclonal
  • Purity: Total IgG. Protein A purified in PBS, 50% glycerol. Filter sterilized.
  • Format: Liquid at 2 mg/ml.
  • Quantity: 100 µg
  • Storage: Store at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
  • Tested applications: ELISA (ELISA), Western blot (WB)
  • Recommended dilutions: assay dependent (ELISA), 1: 1000 - 1: 5000 (WB)
  • Expected | apparent MW: 39 kDa | 43 kDa (chloroplast isoform)
  • Confirmed reactivity: Arabidopsis thaliana, Spinacia oleracea, Zea mays
  • Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Glutamine synthetase plays a role in the flow of nitrogen into nitrogenous organic compounds. There are five root types of GS isoproteis (GS1-1~ GS1-5) and one chloroplastic GS isoprotein (GS2/GLN2) are known. The sequence identity between GS1-1 and GS2 is 65%.
  • Kimata-Ariga and Hase (2014). Multiple complexes of nitrogen assimilatory enzymes in spinach chloroplasts: possible mechanisms for the regulation of enzyme function. PLoS One . 2014 Oct 1;9(10):e108965. doi: 10.1371/journal.pone.0108965. Sakaibara et al. (1996). Molecular identification and characterization of cytosolic isoforms of glutamine synthetase in maize roots. J Biol Chem. 1996 Nov 22;271(47):29561-8. doi: 10.1074/jbc.271.47.29561.
  • No confirmed exceptions from predicted reactivity are currently known

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