Anti-Fd1, Fd2, Fd3, Fd4 | Ferredoxin 1,2,3,4

(Cat#: AS20 4431)
AS20_4431
AS20_4431_1
Description
  • Immunogen: Native, chromafography purified ferredoxin mixture: Fd1, Fd2, Fd3 and Fd4 from Zea mays, UniProt: P27787 (Fd1), P27787(Fd2), P27788 (Fd3), accesion number not known for Fd4
  • Host: Rabbit
  • Clonality: Polyclonal
  • Purity: Total IgG. Protein A purified in PBS, 50% glycerol. Filter sterilized.
  • Format: Liquid at 2 mg/ml.
  • Quantity: 100 µg
  • Storage: Store at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
  • Tested applications: ELISA (ELISA), Western blot (WB)
  • Recommended dilutions: 1: 1000 - 1: 10 000 (WB)
  • Expected | apparent MW: 12 | 16-17 kDa
  • Confirmed reactivity: Arabidopsis thaliana, Zea mays
  • Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions. Occupies a key position both for transferring the photoreducing power to Fd-NADP+ oxidoreductase (FNR), hence the formation of NADPH, and for mediating the cyclic electron flow around photosystem I (PSI).
  • Hase et al. (1991). Molecular Cloning and Differential Expression of the Maize Ferredoxin Gene Family. Plant Physiol. 96(1):77-83. doi: 10.1104/pp.96.1.77.
  • Following processing MW of plant ferredoxins is around 12 kDa, However, due to a strong acidic nature, they migrate at 16 to 17 kDa

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