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{{/_source.additionalInfo}}Anti-BirA (mutated/TurboID) - DyLight® 650 conjugated (40 µg)
(Cat#: AS20 4440-DL650)
Description
- Immunogen: Recombinant mutated BirA protein from E.coli produced using the following plasmid: TurboID-His6_pET21a, (Plasmid #107177). Expression was done in a vector that allowed for the generation of an untagged protein (without HIS6tag).
- Host: Rabbit
- Clonality: Polyclonal
- Purity: Immunogen affinity purified serum, in PBS pH 7.4, conjugated to DyLight® 650.
- Format: Liquid in PBS pH 7,4.
- Quantity: 40 µg
- Storage: Store at 4°C for 12-18 months, A preservative may be added for long time storage up to 2 years. Spin briefly the tube before use.
- Tested applications: Immunofluorescence (IF), Western blot (WB)
- Recommended dilutions: To be determined by end user.
- Expected | apparent MW: Depends upon fusion partner
- Confirmed reactivity: BirA (mutated/TurboID)
- Not reactive in: No confirmed exceptions from predicted reactivity are currently known.
- Tagging a protein with TurboID allows studying protein interactions in different types of cells and organs and developmental stages, This is a suitable tool for proximity labelling experiments as described in Mair et al, (2019), Proximity labelling of protein complexes and cell-type-specific organellar proteomes in Arabidopsis enabled by TurboID, Elife , 2019 Sep 19;8:e47864, doi: 10,7554/eLife,47864, This tag has MW of 35 kDa.
- To be added when available. Antibody released in May 2023.
- DyLight® 650 has Amax = 652 nm, Emax = 672 nm. DyLight® is a registered trademark of Thermofisher Inc., and its subsidiaries.
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