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{{/_source.additionalInfo}}Anti-EGFP, S65T-GFP, RS-GFP, YFP| Green Fluorescence Protein and its variants
(Cat#: AS20 4512)
Description
- Immunogen: Recombinant, His-tagged EGFP protein from Aequorea victoria, UniProt: P42212
- Host: Rabbit
- Clonality: Polyclonal
- Purity: Serum. Contains 0.05 % sodium azide.
- Format: Liquid
- Quantity: 100 µl
- Storage: Store at -20°C, Avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
- Tested applications: Immunofluorescence (IF), Immunoprecipitation (IP), Western blot (WB)
- Recommended dilutions: assay dependent (IP), 1 : 4000 (IF), 1 : 2000 (WB)
- Confirmed reactivity: EGFP, S65T-GFP, RS-GFP, YFP
- GFP (Green fluorescent protein) was originally identified in photo organs on jellyfish Aequorea victoria. It is a naturally fluorescent protein which emits green light at a maximum wavelength of 509 nm when excited by blue or UV light. It is extensively used in laboratory as a reporter molecule to label and study cellular and subcellular proteins in living cells using a wide range of applications. Antibodies to GFP protein are used in immunoblotting and ELISA. GFP protein has molecular weight of 27 kDa.
- Tatsumi et al. (2017). G196 epitope tag system: a novel monoclonal antibody, G196,recognizes the small, soluble peptide DLVPR with high affinity. Sci Rep. 2017 Mar 7;7:43480.doi: 10.1038/srep43480. (Immunofluorescence, Western blot)
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