RecA (protein, positive control)

(Cat#: AS21 4542P)
AS21_4542P
AS21_4542P
Description
  • Purity: Contains 50% glycerol, 20 mM Tris-HCl (pH 8), 1 mM EDTA, 150 mM KCl, 1 mM DTT. Over 90 % pure, by SDS-PAGE
  • Format: Liquid
  • Quantity: 100 µg
  • Storage: Store at -20°C or -80°C for a longer period of time; once make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
  • Tested applications: Western blot (WB)
  • Confirmed reactivity: 38 kDa
  • E. coli RecA protein is a very important enzyme for homologous recombination and recombinational repair. Its synthesis is induced by SOS response caused by DNA damage. RecA protein has multiple functions such as single stranded DNA dependent ATPase activity, DNA annealing activity, formation of D-loop and Holliday structure in homologous recombination reaction, and coprotease activities that promote self-cleavages of LexA repressor, lambda phage repressor and UmuD protein. RecA protein binds to single and double stranded DNA for nucleofilament formation. It carries out a central role in homologous recombination.
  • Ishibashi, Oura S & Umemura (2017) Adsorption of DNA binding proteins to functionalized carbon nanotube surfaces with and without DNA wrapping. Eur Biophys J. 2017 Sep;46(6):541-547. doi: 10.1007/s00249-017-1200-3. Epub 2017 Feb 15. PMID: 28204854.Oura et al. (2015) Biomolecular recognition ability of RecA proteins for DNA on single-walled carbon nanotubes. Colloids Surf B Biointerfaces. 2015 Feb 1;126:496-501. doi: 10.1016/j.colsurfb.2015.01.002. Epub 2015 Jan 10. PMID: 25612818.
  • RecA (protein, positive control)

Boca Scientific is your premiere source for high-quality, innovative solutions for Cell Biology, Molecular Biology, Immunology, genetics and other lab products and reagents. We bring leading-edge products from our own-line and around the world to laboratories in the US and Canada. Our goal is to offer excellent solutions to drive research and discoveries backed by superior customer support.

Recently Viewed Products