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{{/_source.additionalInfo}}Anti-RuvB
(Cat#: AS21 4544)
Description
- Immunogen: Purified, full length, recombinant RuvB protein from E.coli, UniProt: P0A812
- Host: Rabbit
- Clonality: Polyclonal
- Purity: Serum. Contains 0.05 % sodium azide.
- Format: Liquid
- Quantity: 100 µl
- Storage: Store at 4°C for up to 6 monthss, afterwards at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
- Tested applications: Western blot (WB)
- Expected | apparent MW: 1 : 3000 (WB)
- Confirmed reactivity: 37 | 39 kDa
- RuvB protein of Escherichia coli forms a complex with RuvA protein and the complex promotes branch migration of Holliday junction at the late stage of homologous recombination and recombination repair. RuvB is a DNA motor protein which possesses the ATPase activity, activated by DNA and RuvA protein. RuvB in the absence of ATP, it predominantly occurs in a monomer form. In the presence of ATP, it forms dimer and hexamer depending upon the concentration. With RuvA and Holliday junction., it forms a double hexamer.
- Shinagawa & Iwasaki (1996). Processing the holliday junction in homologous recombination. Trends Biochem Sci. 1996 Mar;21(3):107-11. PMID: 8882584.Iwasaki et al. (1992) Escherichia coli RuvA and RuvB proteins specifically interact with Holliday junctions and promote branch migration. Genes Dev. 1992 Nov;6(11):2214-20. doi: 10.1101/gad.6.11.2214. PMID: 1427081.
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