Anti-DNA Photolyase (E,coli)

(Cat#: AS21 4548)
Description
  • Immunogen: Purified, full-length, recombinant DNA photolyase protein from E.coli, UniProt: P00914
  • Host: Rabbit
  • Clonality: Polyclonal
  • Purity: Total IgG. Protein A purified in PBS with 50% glycerol, filter sterilized.
  • Format: Liquid
  • Quantity: 200 µg
  • Storage: Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
  • Tested applications: ELISA (ELISA), Immunoprecipitation (IP), Western blot (WB)
  • Expected | apparent MW: Assay-dependent (ELISA), 1: 200 (IP), 1 : 1000 (WB)
  • Confirmed reactivity: 53.6 kDa
  • Not reactive in: Species of your interest not listed? Contact us
  • Cyclobutane Pyrimidine Dimer photolyase (Deoxyribodipyrimidine photo-lyase) is involved in repair of UV radiation-induced DNA damage. Catalyzes the light-dependent monomerization (300-600 nm) of cyclobutyl pyrimidine dimers (in cis-syn configuration), which are formed between adjacent bases on the same DNA strand upon exposure to ultraviolet radiation. Upon absorption of visible light electrons are transferred from Trp-307 through Trp-360 to Trp 383, and from there to FADH, giving rise to the fully reduced catalytic FADH .

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