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{{/_source.additionalInfo}}Anti-RAD51 | DNA repair protein RAD51 (Saccharomyces cerevisiae) (ChIP grade)
(Cat#: AS21 4549)
Description
- Immunogen: Purified, full length, recombinant and HIS tagged RAD51 protein from Saccharomyces cerevisiae, UniProt: P25454
- Host: Rabbit
- Clonality: Polyclonal
- Purity: Immunogen affinity purified serum in PBS pH 7.4. Contains 50 % glycerol, filter sterilized.
- Format: Liquid
- Quantity: 100 µg
- Storage: Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
- Tested applications: ELISA (ELISA), Chromatin immunoprecipitation (ChIP), Immunofluorescence (IF), Immunoprecipitation (IP), Western blot (WB)
- Expected | apparent MW: 1: 500 : 2000 (WB)
- Confirmed reactivity: 43 kDa
- Not reactive in: Species of your interest not listed? Contact us
- S. cerevisiae Rad 51 protein (400 aa, 43 kDa) is a functional and structural homolog of E.coli RecA and human Rad51 proteins and plays a central role in DNA homologous recombination and recombination repair by promoting homologous DNA strand exchange reaction. Dmcl, Rad55, Rad57 are paralogs of Rad51 and they form complex with Rad51 and Rad52 in mediating recombination processes.
- Muramoto et al. (2018) Phenotypic diversification by enhanced genome restructuring after induction of multiple DNA double-strand breaks. Nat Commun. 2018 May 18;9(1):1995. doi: 10.1038/s41467-018-04256-y. PMID: 29777105; PMCID: PMC5959919. (IF application)Ribeyre & Shore (2012). Anticheckpoint pathways at telomeres in yeast. Nat Struct Mol Biol. 2012 Feb 12;19(3):307-13. doi: 10.1038/nsmb.2225. PMID: 22343724. (ChIP application)
- ChIP application is described in Ribeyre and Shore 2012 while immunofluorescence is described in Muramoto et al 218.
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