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{{/_source.additionalInfo}}Anti-PCNA | Proliferating cell nuclear antigen (Saccharomyces cerevisiae)
(Cat#: AS21 4552)
Description
- Immunogen: Recombinant PCNA protein from Saccharomyces cerevisiae, UniProt: P15873
- Host: Rabbit
- Clonality: Polyclonal
- Purity: Total immunoglobulin fraction in PBS. Contains 50% glycerol, filter sterilized.
- Format: Liquid
- Quantity: 100 µg
- Storage: Store at -20°C and avoid temperature below -25°C ; make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
- Tested applications: Chromatin immunoprecipitation (ChIP), Inmunoprecipitation (IP), Western blot (WB)
- Expected | apparent MW: 1: 100 (IP), 1 : 1000 (WB)
- Confirmed reactivity: 28.9 kDa
- Not reactive in: Species of your interest not listed? Contact us
- This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's accessibility during elongation of the leading strand. Involved in DNA repair and recombination.
- Ogiwara et al. (2007). The INO80 chromatin remodeling complex functions in sister chromatid cohesion. Cell Cycle. 2007 May 2;6(9):1090-5. doi: 10.4161/cc.6.9.4130. Epub 2007 May 8. PMID: 17471029. (ChIP)Hishida et al (2006). Functional and physical interaction of yeast Mgs1 with PCNA: impact on RAD6-dependent DNA damage tolerance. Mol Cell Biol. 2006 Jul;26(14):5509-17. doi: 10.1128/MCB.00307-06. PMID: 16809783; PMCID: PMC1592726. (Western Blot)Iida et al.(2002). PCNA clamp facilitates action of DNA cytosine methyltransferase 1 on hemimethylated DNA. Genes Cells. 2002 Oct;7(10):997-1007. doi: 10.1046/j.1365-2443.2002.00584.x. PMID: 12354094. (Immunoprecipitation)
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