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{{/_source.additionalInfo}}Anti-EGFP | Enhanced Green Fluorescence Protein
(Cat#: AS21 4583)
Description
- Immunogen: Recombinant GFP from Aequorea coerulescens (belt jellyfish) overexpressed and purified from E.coli, UniProt: Q6YGZ0
- Host: Rabbit
- Clonality: Polyclonal
- Purity: Immunogen affinity purified in PBS pH 7.2-7.6, 0.1% trehalose, 5 mM sodium azide.
- Format: Lyophilized
- Quantity: 100 µg
- Reconstitution: For reconstitution add 100 µl of sterile water, ultrapure water
- Storage: Store lypholized antibody at 4 ºC. After reconstitution keep aliquots at -20ºC for a higher stability. Avoid repetitive freeze/thaw cycles. Centrifuge briefly to remove any insoluble material.Expiry date: 12 months after purchase if unopened
- Tested applications: Immunocytochemistry (ICC), Western blot (WB)
- Recommended dilutions: 1:2000 - 1:5000 (ICC), 1:1000 - 1:5000 (WB)
- Expected | apparent MW: 27 kDa (for GFP without fusion partner)
- Confirmed reactivity: GFP, EGFP
- Not reactive in: mCherry
- EGFP is an Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin. Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Xaa-N and Gly-(N+2), and oxidation of Tyr-(N+1) to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.
- To be added when available.
Boca Scientific is your premiere source for high-quality, innovative solutions for Cell Biology, Molecular Biology, Immunology, genetics and other lab products and reagents. We bring leading-edge products from our own-line and around the world to laboratories in the US and Canada. Our goal is to offer excellent solutions to drive research and discoveries backed by superior customer support.