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{{/_source.additionalInfo}}Anti-ACT | Actin (recombinant monoclonal, clone 14H4G8)
(Cat#: AS21 4615)
Description
- Immunogen: Around 100 amino acids of recombinant actin conserved more than 80% in Arabidopsis thaliana: actin-1 P0CJ46 AT2G37620, actin-2 Q96292 AT3G18780, actin-3 P0CJ47 AT3G53750, actin-4 P53494 AT5G59370, actin-5 Q8RYC2 At2g42100, actin-7 P53492 At5g09810, actin-8 Q96293 AT1G49240, actin-11 P53496 AT3G12110, actin-12 P53497 AT3G46520
- Host: Mouse
- Clonality: Monoclonal
- Purity: Immunogen affinity purified serum in PBS pH 7.4.
- Format: Lyophilized
- Quantity: 50 µg
- Reconstitution: For reconstitution add 50 µl, of sterile or deionized water
- Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
- Tested applications: Immunofluorescence (IF), Western blot (WB)
- Recommended dilutions: 1: 500 (IF), 1 : 1000 1: 5000 (WB)
- Expected | apparent MW: 41.6 | 45 kDa
- Confirmed reactivity: Arabidopsis thaliana, Medicago sativa, Zea mays
- Not reactive in: Chlamydomonas reinhardtii
- Actin is a highly conserved protein and an essential component of cell cytoskeleton and plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth. Preferentially expressed in young and expanding tissues, floral organ primordia, developing seeds and emerging inflorescence.
- To be added when available, antibody released in October 2021.
- This antibody is compatible with a secondary antibody for fluorescence detection: goat anti-mouse secondary antibody (IRDye 800CW, Li-cor)
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