Anti-GST tag (mouse monoclonal) (clone S-tag-05)

(Cat#: AS21 4621)
AS21_4621
AS21_4621
Description
  • Immunogen: GST protein
  • Host: Mouse
  • Clonality: Monoclonal
  • Purity: Immunoglobulin purified on Protein A.
  • Format: Liquid at 1 mg/ml.
  • Quantity: 0.1 mg
  • Storage: Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
  • Tested applications: Immunoprecipitation (IP), Western blot (WB)
  • Recommended dilutions: 1 µg/ml (WB)
  • Confirmed reactivity: GST fusion proteins
  • GST-tag (glutathione S-transferase) from a parasite Schistosoma japonicum is a tag added to a protein of interest as a fusion protein for protein purification and detection. It allows purification by affinity chromatography on immobilized glutathione. GST is utilized as a fusion protein with foreign proteins in a range of prokaryotic expression vectors, including the pGEX family of vectors. The tag is 220 amino acid long, ca. 26 kDa which makes it quite large compare to Myc or FLAG tags.
  • To be added when available, antibody released in October 2021.
  • This antibody is recognizing native and denatured fusion proteins containing the GST-Tag sequence expressed in E. coli, yeast, mammalian, and in vitro transcription/translation systems. It can be also used for immuno purification of GST-tagged proteins.

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