Anti-ELF4 | Early flowering 4 (N-terminal)

(Cat#: AS22 4792)
Description
  • Immunogen: KLH-conjugated peptide derived from N-terminal of Arabidopsis thaliana ELF4, UniProt: O04211, TAIR: AT2G40080
  • Host: Rabbit
  • Clonality: Polyclonal
  • Purity: Antigen affinity purified serum, in PBS pH 7.4
  • Format: Lyophilized
  • Quantity: 50 µg
  • Reconstitution: For reconstitution, add 50 µl, of sterile water.
  • Storage: Lyophilized antibody can be stored at -20°C for up to several years. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
  • Tested applications: Western blot (WB)
  • Recommended dilutions: 1 : 1000 (WB)
  • Expected | apparent MW: 12.37 kDa
  • Confirmed reactivity: Arabidopsis thaliana
  • Not reactive in: Colobanthus apetalus, Lupinus luteus, Oryza sativa, Triticum aestivum, Vicia faba
  • ELF4 (Early flowering 4) is the component of the central CCA1/LHY-TOC1 negative feedback loop in the circadian clock that promotes clock accuracy and is required for sustained rhythms in the absence of daily light/dark cycles. Increases ELF3 nuclear distribution and localization in nuclear bodies. ELF4 is necessary for light-induced expression of both CCA1 and LHY and mediates both entrainment to an environmental cycle and circadian rhythm sustainability under constant conditions. Controls flowering time. Alternative name: Protein ARRHYTHMIC 44.
  • To be added when available, antibody available in May 2023.
  • This antibody is recognizing a recombinant 35S::ELF4-GFP. Reactivity in endogenous sample remains to be determined.

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