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{{/_source.additionalInfo}}Bacillus subtilis SURE System Strain, 1ml
Strain NZ8963 (PBS023)
Sales Price$1,452.00
Please note this product requires a license for commercial companies. Please contact us for details.
Description
The SURE® System: Subtilin Regulated gene Expression
Bacillus subtilis is an attractive host for gram-positive bacteria as it is non-pathogenic, does not have significant bias in codon usage, and is capable of secreting functional extracellular proteins directly into the culture medium. It is used in a variety of applications, including agricultural, medical and food biotechnology and the production of recombinant proteins.
Controlled gene expression in B. subtilis is a cost-effective alternative to IPTG induced gene expression. This is achieved with the used of subtilin, a small peptide antibiotic of 32 amino acids produced by B. subtilis. Subtilin production and regulation is encoded in the chromosome by a cluster of 9 genes, which are transcribed from two promoters. Subtilin regulates/activates its own biosynthesis via a two-component regulatory system.
SURE System Strains
- NZ8963 (PBS023) - B. subtilis wild type, subtilin producing strain (ATCC 6633)
- NZ8900 (PBS024) - B. subtilis 168 strain with spaR and spaK integrated into the chromosome at the amyE locus; host strain for subtilin inducible gene expression in B. subtilis
- NZ8901 (PBS025) - B. subtilis 168 with kanamycin gene integrated into the chromosome at the amyE locus; negative control strain
- MC1061 (ELS10610-01) - E. coli strain; intermediate cloning host
Please note these products require a license for commercial companies. Please contact us for licensing information.
- Tightly controlled gene expression in a widely used bacterium
- Complete set of genetic engineering tools available
- Potentially useful for cloning of genes with toxic products
- Can be used for the identification of essential genes after insertion into the chromosome upstream of the gene
- Potential for secretion of gene products
- Controlled gene expression for metabolic engineering
Bacillus subtilis SURE System Vector, 10µg
NICE® E. coli Host Strain MC1061Boca Scientific is your premiere source for high-quality, innovative solutions for Cell Biology, Molecular Biology, Immunology, genetics and other lab products and reagents. We bring leading-edge products from our own-line and around the world to laboratories in the US and Canada. Our goal is to offer excellent solutions to drive research and discoveries backed by superior customer support.