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{{/_source.additionalInfo}}Bacillus subtilis SURE System Vector, 10µg
pNZ8901 (PBS031)
Sales Price$1,288.00
Please note this product requires a license for commercial companies. Please contact us for details.
Description
The SURE® System: Subtilin Regulated gene Expression
Bacillus subtilis is an attractive host for gram-positive bacteria as it is non-pathogenic, does not have significant bias in codon usage, and is capable of secreting functional extracellular proteins directly into the culture medium. It is used in a variety of applications, including agricultural, medical and food biotechnology and the production of recombinant proteins.
Controlled gene expression in B. subtilis is a cost-effective alternative to IPTG induced gene expression. This is achieved with the used of subtilin, a small peptide antibiotic of 32 amino acids produced by B. subtilis. Subtilin production and regulation is encoded in the chromosome by a cluster of 9 genes, which are transcribed from two promoters. Subtilin regulates/activates its own biosynthesis via a two-component regulatory system.
SURE System Vectors
- pNZ8901 (PBS031) - SURE expression vector, PspaSmut, CmR; high promoter activity, but some leakage; suitable for the production of non-toxic proteins.
- pNZ8911 (PBS032) - SURE expression vector, PspaS, CmR; no promoter activity without subtilin induction, lower expression than PspaSmut; suitable for the production of potentially toxic proteins.
- pNZ8902 (PBS033) - SURE expression vector, PspaSmut, EmR; high promoter activity, but some leakage; suitable for the production of non-toxic proteins.
- pNZ8910 (PBS034) - SURE expression vector, PspaS, EmR; some promoter leakage without subtilin induction, caused by the vector; lower expression than pNZ8911.
Several B. subtilis strains specifically developed for the SURE system are available as well. Additionally, E. coli strain MC1061 can be used with the SURE system.
Please note these products require a license for commercial companies. Please contact us for licensing information.
- Tightly controlled gene expression in a widely used bacterium
- Genome sequence available
- Longstanding genetic engineering experience
- Complete set of genetic engineering tools available
- Potentially useful for cloning of genes with toxic products
- Can be used for the identification of essential genes after insertion into the chromosome upstream of the gene in question
- Potential for secretion of gene products
- Controlled gene expression for metabolic engineering
- PBS031 - pNZ8901 SURE System Vector
- PBS032 - pNZ8902 SURE System Vector
- PBS033 - pNZ8910 SURE System Vector
- PBS034 - pNZ8911 SURE System Vector
All vectors are supplied as lyophilized DNA (10µg).
Related Products
Bacillus subtilis SURE System Strain, 1mlBoca Scientific is your premiere source for high-quality, innovative solutions for Cell Biology, Molecular Biology, Immunology, genetics and other lab products and reagents. We bring leading-edge products from our own-line and around the world to laboratories in the US and Canada. Our goal is to offer excellent solutions to drive research and discoveries backed by superior customer support.
