CRISPRi dCas9-KRAB Expression Construct

These ready-to-use dCas9-KRAB expressing constructs ensure optimal expression for transcriptional inhibition. The constructs only contain one selection marker, either antibiotic-resistant gene or fluorescent protein. Multiple promoter options are available enabling flexible CRISPRi applications across a range of cell types. 

Available formats include 11 distinct CRISPRi dCas9-KRAB vector configurations, offered as either 25 µg plasmid DNA or pre-packaged lentiviral particles at 1 × 10^6 TU. Vector options include constitutive dCas9-KRAB expression driven by CMV, EFS, EF1, RSV, or SFFV promoters with selectable markers (Blast, Hygro, Neo, Puro) or fluorescent reporters (TagGFP2, TagRFP).