DNASep™ Ultra Capacity Column

The DNASep™ Ultra Capacity Column is 4.6 mm x 50 mm – For high resolution HPLC ds/ss DNA analysis, sizing, and Mutation Detection. Load capacity of 1.7 mg total DNA or 300 µg Crude DNA.

The DNASep™ Ultra Capacity Column utilizes C18-alkylated polystyrene-divinylbenzene (PS-DVB) copolymer microspheres engineered for enhanced binding capacity and high-performance nucleic acid separations, with a binding capacity up to ~1.7mg. These versatile columns can be used with a variety of HPLC systems for DNA purification..

DNASep Ultra preparative columns are designed specifically for high-performance, scalable purification of RNA and DNA molecules using HPLC-based separation.

Key Specifications
Application: High resolution HPLC ds/ss DNA analysis, sizing, and Mutation Detection
Capacity:  1.7 mg total DNA or 300 µg Crude DNA
Column Dimensions: 4.6 × 50 mm
Column Matrix: PS/DVB resin matrix

DNA Callibration

Liquid chromatography (LC) is a powerful technique for separation and quantitation of nucleic acids due to its high-resolving capability, short analysis time, and ease of recovery of DNA fragments for subsequent studies. Using the DNASep™ Ultra Capacity Column for sizing applications in reversed-phase HPLC provides many advantages. Fragments can be collected and subjected to further molecular analysis such as target verification, sub cloning, amplification and sequencing. DNA fragment sizing is required for quality control (QC) of sequencing templates; quantitation and sizing of DNA fragments, “yes/no” assays for the presence of a DNA fragment in genotyping. Sizing of dsDNA fragments by ion-pair reversed-phase HPLC is largely independent of fragment sequence and sequence related secondary structures of the molecule.