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{{/_source.additionalInfo}}DriverMap™ AIR Spike-In Controls
DriverMap AIR BCR Spike-in Premixed Controls (Human RNA) (Cat#DMAIR-HBR-SC-M)
Description
DriverMap™ AIR Spike-In Controls
Cellecta's DriverMap™ Adaptive Immune Receptor (AIR) RNA Spike-In Controls are synthetic messenger RNA (mRNA) reference standards designed to evaluate, calibrate, and standardize T-cell receptor (TCR) and B-cell receptor (BCR) repertoire sequencing assays. These controls support assessment of assay sensitivity, specificity, quantitative accuracy, sequencing errors, and sample-to-sample contamination, helping researchers improve the reliability and reproducibility of immune clonotype profiling.
The controls consist of 48 synthetic BCR constructs representing 16 receptor isoforms and 39 synthetic TCR constructs representing 13 receptor isoforms. Each isoform includes three distinguishable CDR3 sequence variants within a nearly full-length V(D)J-C receptor structure. Collectively, the controls represent all seven immune receptor chains: TRA, TRB, TRG, and TRD for TCRs, and IGH, IGK, and IGL for BCRs.
Designed for use with multiplex reverse transcription PCR (RT-PCR) and 5′ rapid amplification of cDNA ends (5′-RACE/SMART) PCR-based sequencing methods, the controls are compatible with both commercial and laboratory-developed adaptive immune receptor profiling workflows.
DriverMap AIR RNA Spike-In Controls are available as Premixed Controls or Triplex Isoform Pools. Premixed Controls contain defined concentrations of synthetic receptor variants for evaluating detection sensitivity, quantitative linearity, and sequencing accuracy. Triplex Isoform Pools contain individual receptor isoform pools with distinguishable CDR3 variants, providing flexibility for assay validation, sample quality assessment, and cross-contamination studies.
Fig 1: 48 BCR mRNA spike-in constructs for IGH, IGK and IGL chains are shown. Each construct has three variations in the CDR3 region that differ by three nucleotides in a fixed position.
Fig 2: 39 TCR constructs, each representing unique TRBs, TRAs, TRGs, and TRDs are shown.Each construct has 3 variations in the CDR3 region that differ by 3 nucleotides in fixed positions.
- Comprehensive Receptor Representation: Synthetic RNA constructs collectively represent all four TCR and three BCR receptor chains.
- Defined Reference Sequences: Contains 48 BCR or 39 TCR constructs with known CDR3 sequence variants for standardized assay evaluation.
- Nearly Full-Length Receptor Structures: Incorporates nearly full-length V(D)J-C sequences suitable for evaluating immune receptor amplification and sequencing workflows.
- Pre-Mixed Control Format: Provides three CDR3 variants at defined concentrations of 4,000, 1,000, and 250 molecules/µL, in a 16:4:1 ratio.
- Triplex Isoform Pool Format: Provides 16 BCR or 13 TCR isoform pools, each containing three distinguishable CDR3 variants mixed at a 1:1:1 ratio, with each variant at 2,500 molecules/µL.
- Broad Assay Compatibility: Suitable for evaluating multiplex RT-PCR and 5′-RACE/SMART PCR-based adaptive immune receptor sequencing technologies.
- Quality Control and Standardization: Supports assessment of amplification performance, sequencing errors, sample quality, and cross-contamination.
- Sensitivity and Specificity Assessment: Evaluate detection performance and quantitative linearity using Premixed Controls.
- Error Quantification: Assess errors arising during reverse transcription, PCR amplification, and next-generation sequencing using Premixed Controls.
- Sample Quality Control: Evaluate assay performance and sample quality, including workflows involving degraded RNA or FFPE specimens, using Premixed Controls or Triplex Isoform Pools.
- Cross-Contamination Detection: Identify potential sample-to-sample contamination using distinguishable Triplex Isoform Pools.
- Assay Validation and Standardization: Support performance comparisons across experiments, sequencing runs, and immune repertoire profiling platforms.
|
Cat # |
Description |
Species |
Size |
|
DMAIR-HBR-SC-M |
DriverMap AIR BCR Spike-in Premixed Controls (Human RNA) |
BCR, Mix |
1 kit (for 8 96-Well Plates) |
|
DMAIR-HBR-SC-P |
DriverMap AIR BCR Spike-in Isoform Pools (Human RNA) |
BCR, Pool |
1 kit (for 8 96-Well Plates) |
|
DMAIR-HTR-SC-M |
DriverMap AIR TCR Spike-in Premixed Controls (Human RNA) |
TCR, Mix |
1 kit (for 8 96-Well Plates) |
|
DMAIR-HTR-SC-P |
DriverMap AIR TCR Spike-in Isoform Pools (Human RNA) |
TCR, Pool |
1 kit (for 8 96-Well Plates) |
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