Fast Bst Mix

Fast Bst Mix is a ready-to-use mix containing recombinant DNA polymerase expressed by Geobacillus stearothermophilus. The Bst DNA polymerase displays high strand displacement activity, exhibits 5’ to 3’ polymerase activity, but lacks 5’ to 3’ exonuclease activity. Fast Bst Mix is tolerant to inhibitors, enabling rapid and robust isothermal nucleic acid amplification reactions at a constant temperature. The typical reaction temperature is 65°C. Addition of an intercalating dye allows the reaction to be monitored using a real-time PCR instrument.

Fast Bst Mix is tolerant to inhibitors, enabling rapid and robust LAMP reactions at a constant temperature. The typical reaction temperature is 65°C. However, the enzyme is also active at lower and higher temperatures (55–70°C). The enzyme can be inactivated at temperatures higher than 80°C. Addition of an intercalating dye allows the reaction to be monitored using a real-time PCR instrument. Reactions can also be run using small and portable instruments with incubation and fluorescence measurement capabilities.