PolyMag Neo Magnetofection Reagent is an optimized formulation of PolyMag for a higher gene expression level in primary, hard-to-transfect and cell lines. It is specifically designed for high transfection efficiency. It is composed of magnetic nanoparticles coated with cationic molecules. This reagent must be used with a magnetic plate.

Figure 1: PolyMag Neo transfection efficiency in various cell lines. 1x10⁵ cells were transfected with 0.5 μg / well of pEGFP plasmid DNA in 24-well plates. Transfections were performed with 0.5 μl / well of polyMag Neo reagent. Percentage of transfected cells was measured 24h post transfection by flow cytometry.

Figure 2: PolyMag Neo transfection efficiency in various cell lines. 1x10⁵ cells were transfected with 0.5 μg / well of pEGFP plasmid and 0.5 μl of PolyMag Neo reagent in 24-well plates. EGFP expression was monitored 24h after transfection by fluorescence microscopy.

Features

High transgene expression
High transfection efficiency
Can be used with all types of nucleic acids
Can be used with both primary cells and cell lines
Simple, ready-to-use and rapid protocol
Non-toxic
Compatible with and without serum-containing culture media

Applications

Transfection of primary and hard-to-transfect adherent cells
Nucleic acid delivery
Improving transgene expression

Sizes

PG60100 – PolyMag Neo Nucleic Acid Delivery Reagent, 100 µl (100 transfections)
PG60200 – PolyMag Neo Nucleic Acid Delivery Reagent, 200 µl (200 transfections)
PG61000 – PolyMag Neo Nucleic Acid Delivery Reagent, 1ml (1000 transfections)