PolyMag Neo Magnetofection Reagent is an optimized formulation of PolyMag for a higher gene expression level in primary, hard-to-transfect and cell lines. It is specifically designed for high transfection efficiency. It is composed of magnetic nanoparticles coated with cationic molecules. This reagent must be used with a magnetic plate.
Figure 1: PolyMag Neo transfection efficiency in various cell lines. 1x105 cells were transfected with 0.5 μg / well of pEGFP plasmid DNA in 24-well plates. Transfections were performed with 0.5 μl / well of polyMag Neo reagent. Percentage of transfected cells was measured 24h post transfection by flow cytometry.
Figure 2: PolyMag Neo transfection efficiency in various cell lines. 1x105 cells were transfected with 0.5 μg / well of pEGFP plasmid and 0.5 μl of PolyMag Neo reagent in 24-well plates. EGFP expression was monitored 24h after transfection by fluorescence microscopy.
Features
- High transgene expression
- High transfection efficiency
- Can be used with all types of nucleic acids
- Can be used with both primary cells and cell lines
- Simple, ready-to-use and rapid protocol
- Non-toxic
- Compatible with and without serum-containing culture media
Applications
- Transfection of primary and hard-to-transfect adherent cells
- Nucleic acid delivery
- Improving transgene expression
Sizes
- PG60100 – PolyMag Neo Nucleic Acid Delivery Reagent, 100 µl (100 transfections)
- PG60200 – PolyMag Neo Nucleic Acid Delivery Reagent, 200 µl (200 transfections)
- PG61000 – PolyMag Neo Nucleic Acid Delivery Reagent, 1ml (1000 transfections)