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Transfection for CRISPR

VMC50000 - ViroMag CRISPR Transduction Reagent

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Starting at: $250.00

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ViroMag CRISPR reagent is a viral transduction enhancer for CRISPR/Cas viruses for use in gene editing applications. Based on the Magnetofection™ technology, this reagent concentrates the complete applied dose of viral particles onto cells within minutes, significantly improving virus infectivity with extremely low vector doses. Due to its specific properties, ViroMag CRISPR reagent is also ideal for infecting non-permissive cells. ViroMag CRISPR reagent demonstrates an exceptionally high efficiency to promote, control and assist viral transductions. No molecular biology processes or biochemical modifications are required. 
 
This reagent must be used with a magnetic plate. A starter kit including an 8 x 12 cm magnetic plate is available.
 

Figure 1: ViroMag CRISPR allows high transduction efficiency of CRISPR/Cas9 & gRNA Lentivirus at MOI 2. HeLa, COS7, primary chondrocytes and NIH-3T3 cells were plated in 24-well plates in complete medium at the optimum density of 50-70% confluence at the time of transduction. Lentivirus were added on cells and then incubated overnight at 37oC. GFP and RFP expression was monitored 24h after by fluorescence microscopy.

Features

  • High transduction efficiency in viral CRISPR/Cas9 system
  • Enables genome editing in primary, difficult to transduce and non-permissive cells
  • Accelerates the transduction process
  • Concentrates the viral dose onto the cells
  • Synchronizes cells adsorption/infection without modification of the viruses
 

Applications

  • Genome Editing with CRISPR/Cas9
 

Formats

  • VMC50200 - ViroMag CRISPR reagent, 200 µl
  • VMC51000 - ViroMag CRISPR reagent, 1000 µl
  • KVC50100 - ViroMag CRISPR reagent, 100 µl + Magnetic plate