MagSi-DNA Trial Kit

The MagSi-DNA Trial Kit includes 2ml of each of the 8 types of MagSi-DNA beads for nucleic acid isolation and purification. The beads differ in their bead sizes and surfaces, impacting their separation and suspension time, binding mechanisms, compatible buffer systems, and other factors. With the variety of beads in this kit, the user can determine which beads are ideal for the application.

The following MagSi-DNA beads are included:

• Silica surface, 300 nm bead size (ferromagnetic)
• Carboxylated surface, 300 nm bead size (ferromagnetic)
• Silica surface, 600 nm bead size
• Carboxylated surface, 600 nm bead size
• Silica surface, 1.2 µm bead size
• Carboxylated surface, 1.2 µm bead size
• Silica surface, 3 µm bead size
• Carboxylated surface, 3 µm bead size

MagSi-DNA beads are ideal for purification or isolation of nucleic acids from various sources. The magnetic nanoparticles are intended as a solid phase extraction tool for custom buffer systems based on chaotropic as well non-chaotropic binding principles. These beads can be used for developing your own nucleic acid isolation methods, such as: 

• Isolation of genomic, mitochondrial, or viral DNA from whole blood, cell lysates, human, animal, or plant tissue; isolation of RNA 
• Isolation of genomic, plasmid, or phage DNA from bacterial cultures and bacteria from clinical samples 
• Clean-up of DNA from enzymatic reactions or chromatin immunoprecipitation procedures to remove unwanted substances

MagSi-DNA beads reversibly bind DNA and other nucleic acids under sample- and buffer-specific conditions. A solution containing DNA (e.g. lysate) is combined with the beads and an application-specific binding buffer. After incubation, nucleic acids are bound to the surface. By applying a suitable magnet to the container (tube/deepwell microplate), the bead pellet is separated from the sample mixture. Unwanted components are further removed by washing steps in a selection of buffers. Finally, nucleic acids are released in a DNase/RNase-free water or buffer solution.