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{{/_source.additionalInfo}}Calcein AM, 1 mM solution in anhydrous DMSO
Cat#: MFP-C099
Description
Calcein AM is an ideal fluorescent probe for staining viable cells due to its low cytotoxicity and enhanced hydrophobicity. The hydrophobicity of Calcein AM, unlike Calcein, allows it to readily passes through the cell membrane of viable cells. After Calcein AM permeates into the cytoplasm, it is hydrolyzed by esterases to Calcein, which remains inside the cell.
Calcein does not inhibit any cellular functions such as proliferation or chemotaxis of lymphocytes. Viability assays using Calcein are reliable and correlate well with the standard 51Cr-release assay. The excitation and emission wavelengths of Calcein are 490 nm and 515 nm, respectively.
- Purity = > 90.0% (HPLC)
- Absorbance (100 µl/10 ml 1 M NaOH) = > 0.77 (500 nm)
- Absorbance (100 µl/10 ml DMSO) = < 0.010 (500 nm)
- Low toxicity, enhanced hydrophobicity
- Readily passes through cell membrane of viable cells
- Calcein AM, 1 mM solution in anhydrous DMSO, 1ml
Boca Scientific is your premiere source for high-quality, innovative solutions for Cell Biology, Molecular Biology, Immunology, genetics and other lab products and reagents. We bring leading-edge products from our own-line and around the world to laboratories in the US and Canada. Our goal is to offer excellent solutions to drive research and discoveries backed by superior customer support.
