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{{/_source.additionalInfo}}Anti-HHV-6 IgG (CSF) ELISA kit, 96 tests
(Cat#: ODZ-344)
Description
The ELISA-VIDITEST anti-HHV-6 IgG kit is intended for the serological diagnosis of diseases associated with HHV–6 infection, such as exanthema subitum, acute respiratory illnesses, diarrhea with fever and febrile seizures in infants, heterophile antibody-negative infectious mononucleosis in children, and interstitial pneumonia, encephalitis, meningitis, hepatitis and aplastic anemia in immunodeficient patients. The presence of IgG anti-HHV-6 antibody reveals the immune status of the patient. A significant rise in anti-HHV-6 IgG antibodies in paired serum samples, taken in the acute and convalescent phase of the infection, is indicative of the active infection.
This kit can be used for the anti HHV-6 antibody determination in human sera or cerebrospinal fluids (CSFs). Using quantitative evaluation, the tests can be applied for intrathecal antibody synthesis assessment. The kit does not differentiate between HHV-6 subtype A and B. The assays should be supplemented with determination of anti-HHV-6 IgM antibody in human serum. We recommend the anti-HHV-6 IgM ELISA kit.
The anti-HHV-6 IgG assay is a solid-phase immunoanalytical test that detects IgG antibodies to HHV-6. The strips are coated with native HHV6 antigen. If present in the sera tested, anti-HHV-6 antibodies bind to the immobilized antigens. The antibodies in complexes with antigens are later recognized by animal anti-human IgG antibodies labeled with horseradish peroxidase. The attached labeled antibodies are revealed by an enzymatic reaction with a chromogenic substrate. Negative sera do not react. A mild change in color, if present, may be attributed to the reaction background.
Brief protocol:
- Prepare reagents and samples
- Dispense 100 µl/well standards, control and samples; incubate 60 min. at room temperature; wash 4 times (250 µl/well), aspirate
- Dispense 100 µl/well Px-conjugate; incubate 60 min. at room temperature; wash 4 times (250 µl/well), aspirate
- Dispense 100 µl/well TMB-O substrate; incubate 20 min. at room temperature
- Dispense 100 µl/well Stop solution
- Read the absorbance at 450 / 620-690 nm
- Quantitative evaluation
- Can be used with human sera or cerebrospinal fluids
- High specificity/sensitivity
- Ready-to-use controls and conjugates
- Incubation at laboratory temperature
- Diagnosis of diseases associated with HHV–6 infection
- Intrathecal antibody synthesis assessment
- ELISA strips coated with native antigen, 1 microplate
- Standard A, 1.3ml
- Standard B, 1.3ml
- Standard C, 1.3ml
- Standard D, 1.3ml
- Standard E, 1.3ml
- Anti-human IgG antibodies labeled with horseradish peroxidase (Px-conjugate), 13ml
- Wash buffer 10x concentrate, 125ml
- Dilution buffer anti-HHV6, 100ml
- Chromogenic substrate (TMB substrate), 13ml
- Stop solution, 13ml
Boca Scientific is your premiere source for high-quality, innovative solutions for Cell Biology, Molecular Biology, Immunology, genetics and other lab products and reagents. We bring leading-edge products from our own-line and around the world to laboratories in the US and Canada. Our goal is to offer excellent solutions to drive research and discoveries backed by superior customer support.
