The TurboDoma nutrient mixtures comprise chemically defined, protein and peptide-free minimal culture media for the cultivation of several hybridomas and myeloma cell lines including the authenticated and certified serum-free cell lines SP2/0-TurboDoma, P3X63Ag8.653 - TurboDoma and NSO-TurboDoma available at the European Collection of Animal Cell Cultures. Media for routine maintenance and cryopreservation as well as for mass cell-cultivation and production of monoclonal antibodies and recombinant proteins are offered.