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{{/_source.additionalInfo}}CRISPR sgRNA Cloning Vectors
Lentiviral constructs expressing sgRNA sequences may be transduced into cells with Cas9 or dCas9 variants to knock out, knock down (CRISPRi), or activate (CRISPRa) gene expression in vivo or in vitro.
Cellecta offers a range of constitutive and inducible lentiviral vector backbones with different selection and fluorescent markers for cloning and expression of sgRNA.
If you can’t find a suitable expression construct, we may be able to offer a custom construct.
FAQ's:
1.
Yes, we can provide control constructs in any of our vector backbones.
2.
Most Cellecta vector backbones include markers like RFP or GFP for use in monitoring transduction.
3.
Both these promoters work; EF1α is generally stronger, but UbiC is highly consistent in most mammalian cells.
CRISPR sgRNA Cloning Vectors (linearized)
CRISPR pRSG16-U6-(xx)-UbiC-RFP-2A-Puro (linearized), 500 ng ...