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{{/_source.additionalInfo}}RealMOD™ Green T 2x qRT-PCR mix
100 reactions (Cat#: 25365.100)
Description
The next generation of real-time PCR solutions. RealMOD™ Green T² 2X qRT-PCR Mix combines reverse transcription and qPCR in a single-tube reaction. It includes a 5x gDNA Eraser that 5x gDNA Eraser that rapidly and enzymatically removes contaminating host genomic DNA from RNA templates without compromising transcript stability. The integrated dUTP/UDG system blocks PCR carryover contamination. The mix ensures maximum data integrity for critical research and molecular diagnostics.
The kit contains all the components necessary to perform qRT-PCR: hot-start Taq DNA Polymerase, ultrapure dNTPs/ dUTP mix, Universal ROX reference dye, non-interfering visual tracking dye, SYBR Green I dye and stabilizing reaction buffer in the master mix. Then the 5x gDNA Eraser contains a recombinant sequence-specific endonuclease.
- A comprehensive one-step solution
- 92% target specificity
- Universal ROX that is compatible with any equipment
- Less than40 minutes one-step total runtime
- Built-in, non-interfering blue tracking dye allowing for visual confirmation to reduce errors
- Quantification of cellular and viral RNA Detecting Viruses
- Gene expression analysis with integrated gDNA removal Contents
- High-throughput diagnostic research requiring strict contamination control
- Real-Mod Green T 2 2x qRT-PCR mix*, 1ml, 100 reactions
- Real-Mod Green t 2 2x qRT-PCR mix*, 3ml, 300 reactions
- *Master-Mix contains hot-start Taq DNA Polymerase, ultrapure dNTPs/ dUTP mix, Universal ROX reference dye, non-interfering visual tracking dye, SYBR Green I dye and stabilizing reaction buffer.5x gDNA Eraser contains a recombinant sequence-specific endonuclease optimized for rapid enzymatic degradation of gDNA contains hot-start Taq DNA Polymerase, ultrapure dNTPs/ dUTP mix, Universal ROX reference dye, non-interfering visual tracking dye, SYBR Green I dye and stabilizing reaction buffer.
- Contains hot-start Taq DNA Polymerase, ultrapure dNTPs, MgCl2 and SYBR Green I dye.
