The FluoroStain Protein Fluorescent Staining Dye (Red, 1000x) is designed to substitute the common Coomassie Blue protein staining method, offering greater sensitivity and ease of operation. Unlike Coomassie Blue stain, the FluoroStain Protein Fluorescent Staining Dye binds to proteins with high specificity, making de-staining process an option rather than a requirement. With further reduction of background signals via de-staining process, FluoroStain is capable of achieving detection level parallel to silver staining without specialized imaging equipment, making it one of the most sensitive dyes available.
In addition to its remarkable sensitivity, the FluoroStain Protein Fluorescent Staining Dye brings a more reliable and safe user experience. The stained gel can be visualized with blue-light illumination, avoiding the risk of skin/eye damage caused by UV light. For best results, we suggest using B-BOX Blue Light LED epi-illuminator to visualize and analyze the gel stained with this dye.
When it is bound with bovine serum albumin (BSA), the fluorescent emission of FluoroStain Protein Fluorescent Staining Dye can be excited by UV and blue light sources, with excitation peaks around 369 and 517nm and emission at 605 nm. In the absence of BSA, FluoroStain Protein FluorescentStaining Dye shows ignorable fluorescence as compared with protein-bound form,therefore giving a clear background for photographic analysis.
These spectral characteristics make this fluorescent dyecompatible with a wide variety of gel reading facilities, including UV/ bluelight epi- and transilluminator, argon laser and mercury-arc lamp excitationgel scanners.
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