ReverHotTaq DNA/RNA Polymerase 50x

ReverHotTaq DNA/RNA Polymerase is a new thermostable enzyme with strand displacement and reverse transcription activity for RT-PCR and qPCR.

ReverHotTaq DNA/RNA Polymerase is an artificial engineered thermostable DNA polymerase, which originates from BIORONs SD Polymerase. The HotStart function with AntiTaq antibody prevents non-specific amplification and allows reaction set-up at room temperature. ReverHotTaq shows a high reverse transcriptase activity for sensitive and robust cDNA synthesis and PCR amplification. Therefore, it can be used in a one-step RT-PCR assay with only one enzyme. The enzyme also possesses strand displacement activity and high thermostability up to 93 °C , so it is suitable for RNA templates with a complex secondary structure. Both total RNA and mRNA can be used as template. ReverHotTaq allows to detect target transcripts in a single reaction with only 10 pg of total RNA using gene-specific primers.

ReverHotTaq also provides efficient, robust and specific PCR and qPCR amplification of DNA templates. Due to the 5’ – 3’ exonuclease activity, ReverHotTaq can be used in qPCR or RT-qPCR with hydrolysis probes (e.g. TaqMan). 

Please note, ReverHotTaq Polymerase is not recommended for Isothermal Amplification.

Concentration: 50x (15 U/µl)

Storage: -18 °C to - 22 °C. Avoid freeze-thaw cycles.