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{{/_source.additionalInfo}}RevTaq RT-PCR DNA Polymerase
100 U (Cat#6500S)
Description
RevTaq RT-PCR DNA polymerase is an engineered, extremely thermostable, aptamer-based fast-formulated reverse transcriptase and combined DNA polymerase with a half-life at 95°C of > 40 min. This enzyme allows reverse transcription reactions at high temperatures, minimizing the problems encountered with strong secondary structures in RNA. RevTaq RT-PCR DNA polymerase allows “zero-step” RT-PCRs directly from RNA templates without an isothermal reverse transcription step, as reverse transcription takes place simultaneously with DNA amplification during the cycled PCR elongation step. RevTaq RT-PCR DNA polymerase is the pure, reverse transcription active enzyme ingredient of our Volcano3G® RT-PCR Master Mixes.
Storage: -20°C
Shipping: Cold packs
| References | Blatter, N., Bergen, K., Nolte, O., Welte, W., Diederichs, K., Mayer, J., ... & Marx, A. (2013). Structure and Function of an RNA‐Reading Thermostable DNA Polymerase. Angewandte Chemie International Edition, 52(45), 11935-11939. |
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- Allows “0-step” RTPCRs directly from RNA templates (without an isothermal reverse transcription step)
- Minimizes the problems encountered with strong secondary structures in RNA that melt at elevated temperatures
- Prevents non-specific amplification at ambient temperatures
- End-point PCR
- Real-Time PCR
- #6500S - 100 U:1 x 50 µl RevTaq RT-PCR DNA polymerase (50x); 1 x 1.25 ml 5x Volcano buffer
- #6500M - 500 U: x 250 µ RevTaq RT-PCR DNA polymerase (50x); 2 x 1.25 ml 5x Volcano buffer
Boca Scientific is your premiere source for high-quality, innovative solutions for Cell Biology, Molecular Biology, Immunology, genetics and other lab products and reagents. We bring leading-edge products from our own-line and around the world to laboratories in the US and Canada. Our goal is to offer excellent solutions to drive research and discoveries backed by superior customer support.
