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{{/_source.additionalInfo}}pmIrisFP, FLAG®-tagged
Cat#: VS-FLP10050
Description
pmIrisFP is a cloning and expression vector, encoding for the photoactivatable fluorescent protein mIrisFP.
mIrisFP combines irreversible photoconversion from a green- to a red-emitting form with reversible photo-switching between a fluorescent and a non-fluorescent state in both forms. The protein is an advanced monomeric variant of EosFP that differs from the monomeric mEosFP by four additional mutations: A69V, K145I, F173S and Y189A.
mIrisFP has excellent properties as a genetically encoded fluorescence marker. It matures with a half-life of 14 min at 37°C, faster than many engineered fluorescent proteins in vitro. In HEK293 cells, mIrisFP green fluorescence appears 15h after transfection, similar to EGFP (13h). mIrisFP fluorescence can be switched on and off reversibly, but also allows for the non-reversible photoconversion from green to red. The dual photoactivation capability of mIrisFP offers enhanced flexibilitiy and enables the combination of pulse-chase experiments with super-resolution imaging.
- Expression vector for the fluorescent protein mIrisFP
- Ready-to-use construct for cell tracking
- Green-to-red photoconvertible and photoactivatable
- Permanent, bright and fast
- Monomeric; low tendency for dimer formation
- Suitable for pulse-chase experiments, measurements of kon- and koff- rates, and high-resolution fluorescence microscopy such as PALM
- Source vector for creating fusions with signal peptides
- Selective marking of cells, subcellular compartments and fusion proteins by photoconversion
- Localization studies of fusion proteins with mIrisFP
- Pulse-chase experiments and superresolution imaging with mIrisFP
- pmIrisFP, FLAG-tagged, lyophilized DNA, 10 µg
Boca Scientific is your premiere source for high-quality, innovative solutions for Cell Biology, Molecular Biology, Immunology, genetics and other lab products and reagents. We bring leading-edge products from our own-line and around the world to laboratories in the US and Canada. Our goal is to offer excellent solutions to drive research and discoveries backed by superior customer support.
