phCenpA-15-G, 15ug

(Cat#: VS-KIVA0002)
Description
The vector phCenpA-15-G is a ready-to-use mammalian expression vector encoding a fusion protein of the human kinetochore protein CenpA and the fluorescent protein EGFP. EGFP is fused by a 15 amino acid linker to the C-terminus of hCenpA. phCenpA-15-G can be used to transfect mammalian cell lines, as successfully done for HeLa, HEp-2, and U2OS. EGFP-hCenpA labels centromeres, enabling the application of fluorescent microscopy techniques and the pull-down of inner kinetochore proteins using
GFP-binding proteins.
 
  • Encodes a fusion protein of the human kinetochore protein CenpA and the fluorescent protein EGFP
  • Modern fluorescence microscopy techniques to determine localization, binding, dynamics, protein-protein interactions and protein-protein proximities
  • Fluorescence methods such as FRET, FRAP, FCS, RICS, FCCS, and F3H
  • phCenpA-15-G, lyophilized DNA, 15 µg
 

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