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{{/_source.additionalInfo}}Anti-PsbA | D1 protein of PSII, C-terminal (chicken)
(Cat#: AS01 016)
Description
- Immunogen: KLH-conjugated synthetic peptide derived from available plant, algal and cyanobacterial PsbA sequences, including Arabidopsis thaliana UniProt: A4QJR4, TAIR: AtCg00020 , Oryza sativa P0C434, Populus alba Q14FH6, Physcomitrella patens Q6YXN7, Chlamydomonas reinhardtii P07753, Synechocystis sp. P14660 and many others
- Host: Chicken
- Clonality: Polyclonal
- Purity: Purified, total IgY (chicken egg yolk immunoglobulin) in PBS pH 8. Contains 0.02 % sodium azide.
- Format: Liquid
- Quantity: 100 µl
- Storage: Store at 4°C; make aliquots to avoid working with a stock. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
- Tested applications: Western blot (WB)
- Recommended dilutions: 1 :4000-1 : 8000, 5 µg of total protein, (WB)
- Expected | apparent MW: 38 | 28-30 kDa
- Confirmed reactivity: Alaria esculenta, Amphidinium carterae, Anabaena sp., Arabidopsis thaliana, Brachypodium sylvaticum, Chlamydomonas reinhardtii, Chlamydomonas raudensis (both Antarctic and mesophilic strains), Cyanophora sp. , Cyanothece sp. ATCC 51142, Cynara cardunculus, Gonyaulax polyedra, Fucus vesiculosus, Horderum vulgare, Lobaria pulmonaria, Petunia sp., Pinus sylvestris, Spartina alterniflora, Solanum lycopresicum, Synechococcus sp. PCC 7942, Triticum aestivum, Ulva sp., symbiotic dinoflagellates of Stylophora pistillata and Turbinaria reniformis, Zea mays
- Not reactive in: No confirmed exceptions from predicted reactivity are currently known
- The PsbA (D1) protein of Photosystem II is rapidly cycled under illumination in all oxygenic photobionts. Disruption of PsbA cycling or losses of PsbA pools are central to photoinhibition of photosynthesis in cyanobacteria, algae and plants under a wide range of conditions including excess light, low temperature and UV exposure. Tracking PsbA pools using the Global PsbA antibody can show the functional content of Photosystem II in a wide range of samples.
- Rogowski et al. (2023).Enzymatic kinetics of photosystem II with DCBQ as a substrate in extended Michaelis-Menten model. J Photochem Photobiol B. 2023 Oct:247:112780. doi: 10.1016/j.jphotobiol.2023.112780. Epub 2023 Aug 25. Vitale et al.(2022) Manipulation of light quality is an effective tool to regulate photosynthetic capacity and fruit antioxidant properties of Solanum lycopersicum L. cv. 'Microtom' in a controlled environment. PeerJ. 2022;10:e13677. Published 2022 Jul 1. doi:10.7717/peerj.13677Toubiana et al. (2020). Correlation-based Network Analysis Combined With Machine Learning Techniques Highlight the Role of the GABA Shunt in Brachypodium Sylvaticum Freezing Tolerance. Sci Rep , 10 (1), 4489Sicora et al. (2019). Regulation of PSII function in Cyanothece sp. ATCC 51142 during a light-dark cycle. Photosynth Res. 2019 Mar;139(1-3):461-473. doi: 10.1007/s11120-018-0598-5,Sevilla et al. (2019). Regulation by FurC in Anabaena links the oxidative stress response to photosynthetic metabolism. Plant Cell Physiol. 2019 May 21. pii: pcz094. doi: 10.1093/pcp/pcz094.Figlioli et al. (2019). Overall plant responses to Cd and Pb metal stress in maize: Growth pattern, ultrastructure, and photosynthetic activity. Environ Sci Pollut Res Int. 2019 Jan;26(2):1781-1790. doi: 10.1007/s11356-018-3743-y.
- The antibody is appropriate for detecting both, 24 kDa or the 10 kDa C-terminal fragments, whichever is generated under given treatment conditions.In our analysis we have seen both, ca. 24 kDa and ca. 10 kDa fragments from different samples, depending on treatments and isolation procedures.This antibody will also detect the phosphorylated form of D1as an alternate band to the main band on a high resolution gel.
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