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{{/_source.additionalInfo}}Anti-AtpB | Beta subunit of ATP synthase (chloroplastic + mitochondrial) (chicken antibodies)
(Cat#: AS03 030)
Description
- Immunogen: KLH-conjugated synthetic peptide derived from available plant, algal (chloroplastic and mitochondrial) and bacterial sequences of beta subunits of F-type ATP synthases, including Arabidopsis thaliana chloroplastic ATP synthase subunit beta UniProt: P19366, TAIR: AtCg00480 and Arabidopsis thaliana mitochondrial ATP synthase subunit beta-1, UniProt: P83483, TAIR: At5g08670 as well as Chlamydomonas reinhardtii, UniProt: P06541 and A8IQU3
- Host: Chicken
- Clonality: Polyclonal
- Purity: Purified, total IgY (chicken egg yolk immunoglobulin) in PBS pH 8. Contains 0.02 % sodium azide.
- Format: Liquid at 23.3 µg/µl
- Quantity: 100 µl
- Storage: Store at 2-8°C.; make aliquots to avoid working with a stock. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
- Tested applications: Immunolocalization (IL), Immunogold (IG), Western blot (WB)
- Recommended dilutions: 1 : 500 for localization of native enzyme by immunogold (IL), 1 : 5 000-1 : 8 000 (WB)
- Expected | apparent MW: 53.9 kDa (Arabidopsis thaliana), 51.7 kDa (Synechocystis PCC 6803), 53.7 kDa (Spinacia oleracea)
- Confirmed reactivity: Arabidopsis thaliana, Hordeum vulgare, Phaeodactylum tricornutum, Spartina alterniflora, Spinacia oleracea, Synechocystis PCC 6803, Synechococcus PCC 7942, beef muscle, Rat liver
- Not reactive in: Archeal V-type ATP synthase
- ATP synthase is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient.
- Neusius et al. (2022) Lysine acetylation regulates moonlighting activity of the E2 subunit of the chloroplast pyruvate dehydrogenase complex in Chlamydomonas. Plant J. 2022 Sep;111(6):1780-1800. doi: 10.1111/tpj.15924. Epub 2022 Aug 8. PMID: 35899410.Levitan et al. (2019). Structural and functional analyses of photosystem II in the marine diatom Phaeodactylum tricornutum. Proc Natl Acad Sci U S A. 2019 Aug 27;116(35):17316-17322. doi: 10.1073/pnas.1906726116.Nelson et al. (2019). Protein lysine methylation contributes to modulating the response of sensitive and tolerant Arabidopsis species to cadmium stress. doi: 10.1111/pce.13692.Gellert et al. (2018). A single point mutation on the cucumber mosaic virus surface induces an unexpected and strong interaction with the F1 complex of the ATP synthase in Nicotiana clevelandii plants. Virus Res. 2018 Jun 2;251:47-55. doi: 10.1016/j.virusres.2018.05.005. (immunogold)Quesada et al. (2011). Arabidopsis RUGOSA2 encodes an mTERF family member required for mitochondrion, chloroplast and leaf development. Plant J. Nov;68(4):738-53. doi: 10.1111/j.1365-313X.2011.04726.x. Epub 2011 Sep 13.
- Results of immunogold studies using anti-AtpB antibody are published in Andersson et al. (2009).
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