Anti-LacI | Lactose operon repressor (clone 15C11)

(Cat#: AS16 3144)
Description
  • Immunogen: Recombinant LacI-NLS-6xHis (Lactose operon repressor + C-terminal eukaryotic nuclear localization signal: SSVVHPKKKRKV (NLS) + 6xHis-tag) overexpressed in in E, coli
  • Host: Mouse
  • Clonality: Monoclonal
  • Purity: IgG1 immunoglobulin PBS, 0.05% (w/v) sodium azide.
  • Format: Liquid
  • Quantity: 100 µg
  • Storage: Store at -20°C, Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
  • Tested applications: Western blot (WB)
  • Expected | apparent MW: 5 µg in 10 ml (WB)
  • Confirmed reactivity: 45 kDa
  • Not reactive in: Actinopolyspora erythraea, Chlamydia trachomatis, Methanosarcina mazei, Methyloglobulus morosus KOM1, Pseudomonas sp. 1-7, Shigella sonnei, Shinella sp. Species of your interest not listed? Contact us
  • LacI (Lactose operon repressor) is a DNA-binding protein which represses the expression of genes encoding proteins responsible for lactose metabolism in bacteria. When lactose in absent from the bacterial medium these genes are repressed and when it becomes available it is converted to allolactose which inhibits LacI binding to DNA, thus allowing lactose metabolism.
  • Kim T., et al. (2005) Engineering a root-specific, repressor-operator gene complex. Plant Biotechnol J. 3(6):571-82.
  • This antibody recognizes recombinant and native Lacl

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