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{{/_source.additionalInfo}}Anti-CRD1 | Cyanobacterial homolog of plant CHL27 cyclase
(Cat#: AS06 122)
Description
- Immunogen: residues 1-409 from Arabidopsis thaliana CHL27 fused to TrxA UniProt: Q9M591,TAIR: At3g56940
- Host: Rabbit
- Clonality: Polyclonal
- Purity: Serum
- Format: Lyophilized
- Quantity: 50 µl
- Reconstitution: For reconstitution add 50 µl of sterile water
- Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
- Tested applications: Western blot (WB)
- Recommended dilutions: 1 : 3000 (WB)
- Expected | apparent MW: 47 | 40 kDa (Arabidopsis thaliana)
- Confirmed reactivity: Arabidosps thaliana, Horderum vulgare, Nicotiana tabacum, Pisum sativum, Physcomitrium patens, Chlamydomonas reinhardtii, purple bacteria (CRD1) and (CHL27), Synechocystis PCC 6803, anoxygenic phototrophs (proeobacteria): Congregibacter litoralis, Roseobacter litoralis, green non-sulfur bacterium: Chloroflexus aurantiacus, photosynthetic bacterium: Rubriviavax gelatinosus
- Not reactive in: No confirmed exceptions from predicted reactivity are currently known
- Chl27 catalyzes the cyclase reaction in chlorophyll biosynthesis.
- Hernández et al. (2024). 2-Cys peroxiredoxins contribute to thylakoid lipid unsaturation by affecting ω-3 fatty acid desaturase 8. Plant Physiol. 2024 May 31;195(2):1521-1535. doi: 10.1093/plphys/kiae102. Wang et al. (2020). Post-translational coordination of chlorophyll biosynthesis and breakdown by BCMs maintains chlorophyll homeostasis during leaf development. Nat Commun. 2020; 11: 1254. Cha et al. (2019). Arabidopsis GIGANTEA negatively regulates chloroplast biogenesis and resistance to herbicide butafenacil. Plant Cell Rep. 2019 Jul;38(7):793-801. doi: 10.1007/s00299-019-02409-x.Canniffe et al. (2014). Elucidation of the preferred routes of C8-vinyl reduction in chlorophyll and bacteriochlorophyll biosynthesis. Biochem J. 2014 Jun 19.Lang et al. (2011).Simultaneous isolation of pure and intact chloroplasts and mitochondria from moss as the basis for sub-cellular proteomics. Plant Cell Rep. 2011 Feb;30(2):205-15.doi: 10.1007/s00299-010-0935-4.
- Antibodies detect two isoforms in Chlamydomonas reinhardtii, CRD1 in cells grown under copper deficiency (39.8 kDa) and CTH1 in cells grown with sufficient copper (40.7 kDa). Antibodies will also react with Arabidopsis thaliana, Hordeum vulgare, Pisum sativum, and purple bacteria
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