Anti-SPS | Sucrose phosphate synthase (maize)

(Cat#: AS06 185)
AS06_185
AS06_185_1
Description
  • Immunogen: Synthetic peptide derived from Zea mays SPS protein sequence (P31927).
  • Host: Rabbit
  • Clonality: Polyclonal
  • Purity: Total IgG. Protein G purified in PBS pH 7.4.
  • Format: Lyophilized
  • Quantity: 50 µl
  • Reconstitution: For reconstitution add 50 µl of sterile water
  • Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
  • Tested applications: Immunolocalization (IL), Western blot (WB)
  • Recommended dilutions: 1 : 1500 (IL), 1 : 2000 (WB)
  • Expected | apparent MW: 120 | ~130 for Zea mays
  • Confirmed reactivity: Alfalfa, Solanum lycopersicum, Zea mays
  • Not reactive in: Hordeum vulgare
  • SPS (sucrose phosphate synthase, EC 2.4.1.14) is the key enzyme of carbon flux into sucrose fixation in plants. It catalyzes the synthesis of sucrose-phosphate from UDP-glucose and fructose-6-phosphate predominantly in the cytosol of sucrose-source leaf tissue.
  • Padhi et al. (2019). Distinct nodule and leaf functions of two different sucrose phosphate synthases in alfalfa. Planta (2019). https://doi.org/10.1007/s00425-019-03261-9.Kaur et al. (2019). Comparison of alfalfa plants overexpressing glutamine synthetase with those overexpressing sucrose phosphate synthase demonstrates a signaling mechanism integrating carbon and nitrogen metabolism between the leaves and nodules. Plant Direct, Volume3, Issue1, Jan 2019, e00115.Seger et al. (2014). Impact of concurrent overexpression of cytosolic glutamine synthetase (GS 1 ) and sucrose phosphate synthase (SPS) on growth and development in transgenic tobacco. Planta. 2014 Sep 12.Rounis et al. (2014). Seeded and Parthenocarpic Cherry Tomato Fruits Exhibit Similar Sucrose, Glucose, and Fructose Levels, Despite Dissimilarities in UGPase and SPS Gene Expression and Enzyme Activity. J Plant Growth Regul. DOI 10.1007/s00344-014-9441-1. (immunolocalization)

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