Anti-FtsH4 | ATP-dependent zinc metalloprotease FtsH4 (mitochondrial)

(Cat#: AS07 205)
AS07_205
AS07_205_1
Description
  • Immunogen: KLH-conjugated peptide derived from sequence of Arabidopsis thaliana FtsH4 UniProt: O80983, TAIR: At2g26140
  • Host: Rabbit
  • Clonality: Polyclonal
  • Purity: Immunogen affinity purified serum in PBS pH 7.4.
  • Format: Lyophilized
  • Quantity: 200 µg
  • Reconstitution: For reconstitution add 100 µl of sterile water
  • Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
  • Tested applications: Western blot (WB)
  • Recommended dilutions: 1 : 500 (WB)
  • Expected | apparent MW: 77 | 72 kDa
  • Confirmed reactivity: Arabidopsis thaliana, Brassica oleracea var. botrytis
  • Not reactive in: Solanum lycopersicum
  • One of the several classes of mitochondrial proteases is membrane bound, ATPdependent FtsH protease. Their function is very important for the control of protein quality and quantity by degradation of unassembled subunits. Synonyme: cell division protease ftsH homolog 4, mitochondrial
  • Opalińska et al. (2017). Identification of Physiological Substrates and Binding Partners of the Plant Mitochondrial Protease FTSH4 by the Trapping Approach. Int J Mol Sci. 2017 Nov 18;18(11). pii: E2455. doi: 10.3390/ijms18112455.Dolzblasz et al. (2016). The mitochondrial protease AtFTSH4 safeguards Arabidopsis shoot apical meristem function. Sci Rep. 2016 Jun 20;6:28315. doi: 10.1038/srep28315.Rurek et al. (2015). Biogenesis of mitochondria in cauliflower (Brassica oleracea var. botrytis) curds subjected to temperature stress and recovery involves regulation of the complexome, respiratory chain activity, organellar translation and ultrastructure. Biochim Biophys Acta. 2015 Jan 21. pii: S0005-2728(15)00016-X. doi: 10.1016/j.bbabio.2015.01.005.Zhang et al. (2014). Perturbation of auxin homeostasis caused by mitochondrial FtSH4 gene-mediated peroxidase accumulation regulates Arabidopsis architechture. Mol Plant. 2014 Jan 30.Kwasniak et al. (2013). Silencing of the Nuclear RPS10 Gene Encoding Mitochondrial Ribosomal Protein Alters Translation in Arabidiopsis Mitochondria. Plant Cell, May 30.

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